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cd86 monoclonal antibody (it2.2)-pe  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd86 monoclonal antibody (it2.2)-pe
    Cd86 Monoclonal Antibody (It2.2) Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe-cd86+it2%2E2+antibody/cd86+antibody/pm40549286-67-8-16
    Average 90 stars, based on 1 article reviews
    cd86 monoclonal antibody (it2.2)-pe - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Knockdown:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Activation Assay:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Expressing:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Virus:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Incubation:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Quantitative RT-PCR:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Flow Cytometry:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Labeling:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Fluorescence:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Standard Deviation:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Neutralization:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Control:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Cell Culture:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Co-Culture Assay:

    Article Title: Dendritic cells maturated by co-culturing with HIV-1 latently infected Jurkat T cells or stimulating with AIDS-associated pathogens secrete TNF-α to reactivate HIV-1 from latency
    Article Snippet: To characterize cell phenotype, the following antibodies or isotype-matched IgG were used for flow cytometry (clone numbers and sources were given in parentheses): APC-Alexa Fluor750-CD11c (B-ly6; BD Biosciences PharMingen); phycoerythrin (PE)-CD14 (61D3; eBioscience); PE-CD83 (H1B19; eBioscience); PE-CD86 (IT2.2; eBioscience), APC-cy7-HLA-DR (LN3; eBioscience) and PE-CD40 (KPL-1; eBioscience).

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell
    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).



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    Thermo Fisher cd86-pe antibody it2.2
    Mucosal immune response of BEC/DC/T cell upon HDM exposure. (A) A schematic overview of the coculture steps in this human in vitro bronchial mucosal immune model (adapted from ). Calu-3 bronchial epithelial cells (BEC) were cultured for 14 days in air-liquid interface (ALI) prior to coculture with monocyte-derived dendritic cells (moDCs). BEC-DCs and DCs alone were apically exposed to 10 μg/ml HDM for 24 h. After 24 h of exposure to HDM, primed DCs were collected for analysis and coculture with allogenic naïve Th cells for 5 days. Upon 24 h of HDM exposure, supernatants and moDCs were collected to measure secreted levels of (B) IL33, (C) TSLP, (D) TGFβ, (E) IL8 and determine the percentage of moDCs expressing the costimulatory markers (F) CD80 and (G) <t>CD86.</t> After subsequent coculture with of primed DCs with naïve Th cells, supernatants and cells were collected to measure the percentage of (H) CRTH2 and (I) IL13 expressing cells and secreted (J) IL4 as part of the T helper 2 cell response. In addition, the T helper 1 response was analyzed based on the percentage of (K) CXCR3 and (L) IFNγ expressing cells and secreted (M) IFNγ. The regulatory T cell response was determined by identification of the percentage of dual expressing (N) FoxP3 and CD25 cells and secretion of (O) IL10. Finally, (P) the ratio of type 2 IL4 and type 1 IFNγ secretion was calculated. Data is analyzed by paired t -tests, n = 6 biologically different donors (2 independent experiments each using 3 different donors), mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001). Some parts of this figure were created with BioRender.com
    Cd86 Pe Antibody It2.2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GPH fiber regulates the polarization of macrophages. ( A ) Confocal laser images of macrophages after different treatments with LPS, extract in 2 days and extract in 7 days. ( B and C ) Quantization of <t>CD86</t> and CD 206 fluorescence intensity in confocal. ( D ) The concentration of TGF-β, TNF-α and IL-10 in cell supernatant of different groups. The data are shown as the means ± standard deviation from three independent experiments. ** P < 0.01, *** P < 0.001 indicates significant differences between the indicated columns.
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    GPH fiber regulates the polarization of macrophages. ( A ) Confocal laser images of macrophages after different treatments with LPS, extract in 2 days and extract in 7 days. ( B and C ) Quantization of <t>CD86</t> and CD 206 fluorescence intensity in confocal. ( D ) The concentration of TGF-β, TNF-α and IL-10 in cell supernatant of different groups. The data are shown as the means ± standard deviation from three independent experiments. ** P < 0.01, *** P < 0.001 indicates significant differences between the indicated columns.
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    GPH fiber regulates the polarization of macrophages. ( A ) Confocal laser images of macrophages after different treatments with LPS, extract in 2 days and extract in 7 days. ( B and C ) Quantization of <t>CD86</t> and CD 206 fluorescence intensity in confocal. ( D ) The concentration of TGF-β, TNF-α and IL-10 in cell supernatant of different groups. The data are shown as the means ± standard deviation from three independent experiments. ** P < 0.01, *** P < 0.001 indicates significant differences between the indicated columns.
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    GPH fiber regulates the polarization of macrophages. ( A ) Confocal laser images of macrophages after different treatments with LPS, extract in 2 days and extract in 7 days. ( B and C ) Quantization of <t>CD86</t> and CD 206 fluorescence intensity in confocal. ( D ) The concentration of TGF-β, TNF-α and IL-10 in cell supernatant of different groups. The data are shown as the means ± standard deviation from three independent experiments. ** P < 0.01, *** P < 0.001 indicates significant differences between the indicated columns.
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    Image Search Results


    Naf1 knockdown increases NF-κB activation and cytokines expression. A Naf1 knockdown in MDDCs during the treatment with pseudo-typed HIV-luc/JRFL (10 ​ng p24 gag amount virus). B Expressions of cytokines and chemokines. MDDCs (5 ​× ​10 5 ​cells) with or without Naf1 knockdown were incubated in presence or absence of LPS (10 ​ng/mL) for 6 ​h, and cell total mRNAs were extracted, and the cytokines and chemokines were measured with real-time (RT-) PCR. Data are presented as mean ​± ​SD from three representative experiments. ∗∗∗ P ​< ​0.001 was considered as significant difference in unpaired t -test. C, D MDDCs (5 ​× ​10 5 ​cells) with or without Naf1 knockdown were used to detect the surface expressions of CD83, CD86 and HLA-DR with flow cytometry, and results from three donors were summarized ( D ).

    Journal: Virologica Sinica

    Article Title: Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4 + T cell

    doi: 10.1016/j.virs.2025.03.007

    Figure Lengend Snippet: Naf1 knockdown increases NF-κB activation and cytokines expression. A Naf1 knockdown in MDDCs during the treatment with pseudo-typed HIV-luc/JRFL (10 ​ng p24 gag amount virus). B Expressions of cytokines and chemokines. MDDCs (5 ​× ​10 5 ​cells) with or without Naf1 knockdown were incubated in presence or absence of LPS (10 ​ng/mL) for 6 ​h, and cell total mRNAs were extracted, and the cytokines and chemokines were measured with real-time (RT-) PCR. Data are presented as mean ​± ​SD from three representative experiments. ∗∗∗ P ​< ​0.001 was considered as significant difference in unpaired t -test. C, D MDDCs (5 ​× ​10 5 ​cells) with or without Naf1 knockdown were used to detect the surface expressions of CD83, CD86 and HLA-DR with flow cytometry, and results from three donors were summarized ( D ).

    Article Snippet: Monoclonal anti-human antibodies against the following molecules were used in immunostaining: PE-CD83 (HB15e, eBioscience, San Diego, CA, USA); PE-CD86 (IT2.2, eBioscience); APC-cy7-HLA-DR (LN3, eBioscience); PE-ICAM-1 (HA58, eBioscience); PerCP-cy5.5-CD3 (OKT3, eBioscience) and PE-CD69 (FN50, eBioscience).

    Techniques: Knockdown, Activation Assay, Expressing, Virus, Incubation, Quantitative RT-PCR, Flow Cytometry

    Mucosal immune response of BEC/DC/T cell upon HDM exposure. (A) A schematic overview of the coculture steps in this human in vitro bronchial mucosal immune model (adapted from ). Calu-3 bronchial epithelial cells (BEC) were cultured for 14 days in air-liquid interface (ALI) prior to coculture with monocyte-derived dendritic cells (moDCs). BEC-DCs and DCs alone were apically exposed to 10 μg/ml HDM for 24 h. After 24 h of exposure to HDM, primed DCs were collected for analysis and coculture with allogenic naïve Th cells for 5 days. Upon 24 h of HDM exposure, supernatants and moDCs were collected to measure secreted levels of (B) IL33, (C) TSLP, (D) TGFβ, (E) IL8 and determine the percentage of moDCs expressing the costimulatory markers (F) CD80 and (G) CD86. After subsequent coculture with of primed DCs with naïve Th cells, supernatants and cells were collected to measure the percentage of (H) CRTH2 and (I) IL13 expressing cells and secreted (J) IL4 as part of the T helper 2 cell response. In addition, the T helper 1 response was analyzed based on the percentage of (K) CXCR3 and (L) IFNγ expressing cells and secreted (M) IFNγ. The regulatory T cell response was determined by identification of the percentage of dual expressing (N) FoxP3 and CD25 cells and secretion of (O) IL10. Finally, (P) the ratio of type 2 IL4 and type 1 IFNγ secretion was calculated. Data is analyzed by paired t -tests, n = 6 biologically different donors (2 independent experiments each using 3 different donors), mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001). Some parts of this figure were created with BioRender.com

    Journal: Frontiers in Nutrition

    Article Title: HMOS 2’FL and 3FL prevent house dust mite induced proinflammatory cytokine release in vitro and decrease specific IgE production in a murine allergic asthma model

    doi: 10.3389/fnut.2025.1491430

    Figure Lengend Snippet: Mucosal immune response of BEC/DC/T cell upon HDM exposure. (A) A schematic overview of the coculture steps in this human in vitro bronchial mucosal immune model (adapted from ). Calu-3 bronchial epithelial cells (BEC) were cultured for 14 days in air-liquid interface (ALI) prior to coculture with monocyte-derived dendritic cells (moDCs). BEC-DCs and DCs alone were apically exposed to 10 μg/ml HDM for 24 h. After 24 h of exposure to HDM, primed DCs were collected for analysis and coculture with allogenic naïve Th cells for 5 days. Upon 24 h of HDM exposure, supernatants and moDCs were collected to measure secreted levels of (B) IL33, (C) TSLP, (D) TGFβ, (E) IL8 and determine the percentage of moDCs expressing the costimulatory markers (F) CD80 and (G) CD86. After subsequent coculture with of primed DCs with naïve Th cells, supernatants and cells were collected to measure the percentage of (H) CRTH2 and (I) IL13 expressing cells and secreted (J) IL4 as part of the T helper 2 cell response. In addition, the T helper 1 response was analyzed based on the percentage of (K) CXCR3 and (L) IFNγ expressing cells and secreted (M) IFNγ. The regulatory T cell response was determined by identification of the percentage of dual expressing (N) FoxP3 and CD25 cells and secretion of (O) IL10. Finally, (P) the ratio of type 2 IL4 and type 1 IFNγ secretion was calculated. Data is analyzed by paired t -tests, n = 6 biologically different donors (2 independent experiments each using 3 different donors), mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001). Some parts of this figure were created with BioRender.com

    Article Snippet: Titrated amounts of the following antibodies were used to stain in vitro samples: CD11c-PerCP (3.9), HLA-DR-PE (LN3), CD80-FITC (2D10.4), CD86-PE/Cy7 (IT2.2) (All from eBioscience, USA), CD4-PerCP (OKTO4, eBioscience), CXCR3-Alexa Fluor 488 (1C6/CXCR3, BD Biosciences), CRTH2-APC (BM16, BD Biosciences), FoxP3-eFluor 660 (PCH101, Invitrogen), CD25-Alexa Fluor 488 (BC96, eBioscience), and IL13-PE (85BRD, eBioscience), and IFNγ-Amcyan (4S.B3, Biolegend).

    Techniques: In Vitro, Cell Culture, Derivative Assay, Expressing

    The immunomodulatory effects of HMOS were tested in this human in vitro bronchial mucosal immune model by preincubation of HMOS prior to HDM exposure. (A) Calu-3 BECs were cultured in ALI for 14 days before coculture with moDCs and basolateral preincubation with 0,01-0,05% 2’FL or 3FL for 24 h. Next, BEC/DC were apically exposed to 10 μg/ml HDM for 24 h. Followed by a coculture of primed moDCs with naïve Th cells for 5 days. After HMOS preincubation and HDM exposure, primed DCs were collected for analysis and coculture with allogenic naïve Th cells for 5 days (adapted from . Upon HMOS preincubation and HDM exposure, supernatants and moDCs were collected to measure secreted levels of (B) IL33, (C) TSLP, (D) TGFβ, (E) IL8 and determine the percentage of moDCs expressing the costimulatory markers (F) CD80 and (G) CD86. After subsequent coculture with of primed DCs with naïve Th cells, supernatants and cells were collected to measure the percentage of (H) CRTH2 and (I) IL13 expressing cells and secreted (J) IL4 as part of the T helper 2 cell response. In addition, the T helper 1 response was analyzed based on the percentage of (K) CXCR3 and (L) IFNγ expressing cells and secreted (M) IFNγ. The regulatory T cell response was determined by identification of the percentage of dual expressing (N) FoxP3 and CD25 cells and secretion of (O) IL10. Finally, (P) the ratio of type 2 IL4 and type 1 IFNγ secretion was calculated. When data fit a normal distribution and had an equal variability of differences, analysis was performed by One-Way ANOVA followed by a Dunnett’s multiple comparisons test. When these criteria were not met, data was transformed, a Geisser–Greenhouse correction was performed or a Friedman-test with Dunn’s multiple comparisons test was performed, n = 6 biologically different donors (2 independent experiments each using 3 different donors, mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001). Controls of are the same as those depicted in . Some parts of this figure were created with BioRender.com

    Journal: Frontiers in Nutrition

    Article Title: HMOS 2’FL and 3FL prevent house dust mite induced proinflammatory cytokine release in vitro and decrease specific IgE production in a murine allergic asthma model

    doi: 10.3389/fnut.2025.1491430

    Figure Lengend Snippet: The immunomodulatory effects of HMOS were tested in this human in vitro bronchial mucosal immune model by preincubation of HMOS prior to HDM exposure. (A) Calu-3 BECs were cultured in ALI for 14 days before coculture with moDCs and basolateral preincubation with 0,01-0,05% 2’FL or 3FL for 24 h. Next, BEC/DC were apically exposed to 10 μg/ml HDM for 24 h. Followed by a coculture of primed moDCs with naïve Th cells for 5 days. After HMOS preincubation and HDM exposure, primed DCs were collected for analysis and coculture with allogenic naïve Th cells for 5 days (adapted from . Upon HMOS preincubation and HDM exposure, supernatants and moDCs were collected to measure secreted levels of (B) IL33, (C) TSLP, (D) TGFβ, (E) IL8 and determine the percentage of moDCs expressing the costimulatory markers (F) CD80 and (G) CD86. After subsequent coculture with of primed DCs with naïve Th cells, supernatants and cells were collected to measure the percentage of (H) CRTH2 and (I) IL13 expressing cells and secreted (J) IL4 as part of the T helper 2 cell response. In addition, the T helper 1 response was analyzed based on the percentage of (K) CXCR3 and (L) IFNγ expressing cells and secreted (M) IFNγ. The regulatory T cell response was determined by identification of the percentage of dual expressing (N) FoxP3 and CD25 cells and secretion of (O) IL10. Finally, (P) the ratio of type 2 IL4 and type 1 IFNγ secretion was calculated. When data fit a normal distribution and had an equal variability of differences, analysis was performed by One-Way ANOVA followed by a Dunnett’s multiple comparisons test. When these criteria were not met, data was transformed, a Geisser–Greenhouse correction was performed or a Friedman-test with Dunn’s multiple comparisons test was performed, n = 6 biologically different donors (2 independent experiments each using 3 different donors, mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001). Controls of are the same as those depicted in . Some parts of this figure were created with BioRender.com

    Article Snippet: Titrated amounts of the following antibodies were used to stain in vitro samples: CD11c-PerCP (3.9), HLA-DR-PE (LN3), CD80-FITC (2D10.4), CD86-PE/Cy7 (IT2.2) (All from eBioscience, USA), CD4-PerCP (OKTO4, eBioscience), CXCR3-Alexa Fluor 488 (1C6/CXCR3, BD Biosciences), CRTH2-APC (BM16, BD Biosciences), FoxP3-eFluor 660 (PCH101, Invitrogen), CD25-Alexa Fluor 488 (BC96, eBioscience), and IL13-PE (85BRD, eBioscience), and IFNγ-Amcyan (4S.B3, Biolegend).

    Techniques: In Vitro, Cell Culture, Expressing, Transformation Assay

    GPH fiber regulates the polarization of macrophages. ( A ) Confocal laser images of macrophages after different treatments with LPS, extract in 2 days and extract in 7 days. ( B and C ) Quantization of CD86 and CD 206 fluorescence intensity in confocal. ( D ) The concentration of TGF-β, TNF-α and IL-10 in cell supernatant of different groups. The data are shown as the means ± standard deviation from three independent experiments. ** P < 0.01, *** P < 0.001 indicates significant differences between the indicated columns.

    Journal: Regenerative Biomaterials

    Article Title: Bioactive fibrous scaffolds with programmable release of polypeptides regulate inflammation and extracellular matrix remodeling

    doi: 10.1093/rb/rbad010

    Figure Lengend Snippet: GPH fiber regulates the polarization of macrophages. ( A ) Confocal laser images of macrophages after different treatments with LPS, extract in 2 days and extract in 7 days. ( B and C ) Quantization of CD86 and CD 206 fluorescence intensity in confocal. ( D ) The concentration of TGF-β, TNF-α and IL-10 in cell supernatant of different groups. The data are shown as the means ± standard deviation from three independent experiments. ** P < 0.01, *** P < 0.001 indicates significant differences between the indicated columns.

    Article Snippet: Tβ4 (Purity = 99.2%) and aprotinin (lyophilized powder, 3–8 TIU/mg solid, 95%) are purchased in Sigma-Aldrich (St. Louis, MO, USA), the antibody of CD86 (PE, IT2.2), CD206 (FITC, C068C2) are purchased from thermos Fisher Scientific (81WymanStreet, Waltham, MA, USA), PCL (Mw = 86000 Da), HA (40–100 KDa) and gelatin (Pharmaceutical grade, glue strength ∼240 bloom) are purchased in Energy Chemical (Shanghai, China).

    Techniques: Fluorescence, Concentration Assay, Standard Deviation